An Adaptation of Folin's Method for the Quantitative Determination of Creatine and Creatinine (Classic Reprint)

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Excerpt from An Adaptation of Folin's Method for the Quantitative Determination of Creatine and CreatinineThe quantitative method for the determination of creat ine and creatinine, which was perfected by Folig? Is the most accurate which we possess to-day. This method is based on the werk of Jafféf'who showed that when a solution containing creat inine is treated with a solution of picric acid and made alka line with sodium hydroxide a beafiiful deep, red -orange color is produced. Previous to this time creatinine was precipitated with zinc chloride, filtered, and weighed as the double salt of creat inine and zinc chloride. Folin's method was perfected for the use in the determination of creatinine in urines. The method is as follows: Place ten cubic centimeters of urine in a 500 cc. Volumetric flask, add 15cc. Of a saturated solution of picric acid and Sec. Of a 10 per cent solution of sodium hydroxide, then shake thoroughly, and allow the mixture to stand. After exactly five minutes, dilute the contents of the flask to the 500cc. Mark, and pour some of the solution ants one of the cylinders of the Duboscdrcolorimeter. One of the cylinders of the colorimeter is filled with a half-normal solution of potassium dichromate and the prism plunged into it until the scale reads 8mm. In the one containing the solution of creatinine, picric acid, and sodium hydroxide the prism plunger is adjusted until there is an equal depth of color in the two halves of the field at the eye piece. Four readings should be made in each determination and the average used as the final result. From such data, the amount.About the PublisherForgotten Books publishes hundreds of thousands of rare and classic books. Find more at www.forgottenbooks.comThis book is a reproduction of an important historical work. Forgotten Books uses state-of-the-art technology to digitally reconstruct the work, preserving the original format whilst repairing imperfections present in the aged copy. In rare cases, an imperfection in the original, such as a blemish or missing page, may be replicated in our edition. We do, however, repair the vast majority of imperfections successfully, any imperfections that remain are intentionally left to preserve the state of such historical works.